Los alamos daily post circulation8/15/2023 ![]() However, there is an increasing use of peptide ligands with radiometals for specific indications to delineate molecularly specific disease types. The majority of investigations use the metabolic tracer 18F-fluorodeoxyglucose ( 18F-FDG). Positron emission tomography (PET) is well-established in oncologic radiological workflows to detect and monitor disease progression. Stable 44Sc/ 47Sc-complexation will be more favorable for in vivo use and for clinical utility. Our data demonstrated that free 44ScCl 3 remained in the circulation for extended periods, which resulted in approximately 10 times greater radiation burden than stably chelated 44Sc. ConclusionsĪ clinical-scale 44Ti/ 44Sc generator system with a modular design was developed to supply 44ScCl 3 in 0.05 M HCl, which is suitable for further radiolabeling and in vivo use. Liver, spleen and heart wall will receive the highest absorbed dose: 0.524, 0.502, and 0.303 mGy/MBq, respectively, for the adult male. The dose estimation of 44ScCl 3 reveals that the radiation burden is 0.146 mSv/MBq for a 70 kg adult male and 0.179 mSv/MBq for a 57 kg adult female. In vivo imaging and ex vivo distribution studies of the reversible modular generator for 44ScCl 3, -citrate and -NODAGA show that free 44Sc remained in the circulation significantly longer than the chelated 44Sc. Run in forward and reverse directions, the 44Sc/ 44Ti ratio from a primary column was significantly improved from 1038 ± 440 to 3557 ± 680 (Bq/Bq) when a secondary, replaceable, ZR resin cartridge was employed at the flow outlet. Estimated generator capacity was computed based on elution time interval and the total amount of 44Ti loaded on the generator. After loading and initial stabilization, 44ScCl 3 was eluted with 0.05 M HCl in typical yields of 82.9 ± 5.3% ( N = 16), which was normalized to the estimated generator capacity. ResultsĤ4Ti in 6 M HCl was loaded onto the organic resin generator with a yield of 99.97%. Based on the biodistribution data, absorbed doses of 44/47ScCl 3 in human adults were calculated for 18 organs and tissues using the IDAC-Dose software. ![]() After assembly, 44Sc was eluted with 0.05 M HCl for further PET imaging and biodistribution studies in female Swiss Webster mice. ![]() ![]() MethodsĤ4Ti/ 44Sc (101.2 MBq) in 6 M HCl solution was utilized to assemble a modular ZR resin containing generator. In our current study, we built and evaluated a novel clinical-scale 44Ti/ 44Sc generator, explored the pharmacokinetic profiles of 44ScCl 3, -citrate and -NODAGA (1,4,7-triazacyclononane,1-glutaric acid-4,7-acetic acid) in naïve mice, and estimated the radiation burden of 44ScCl 3 in humans. Early animal and patient studies have demonstrated the utility of 44Sc. The 44Ti/ 44Sc generator allows daily onsite production of this diagnostic isotope, which may provide an attractive alternative for PET facilities that lack in-house irradiation capabilities. 44Sc/ 47Sc is an attractive theranostic pair for targeted in vivo positron emission tomographic (PET) imaging and beta-particle treatment of cancer. ![]()
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